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Acid Hemoglobins Electrophoresis Kit

The Microgel/Interlab G26 Acid Hemoglobins Electrophoresis method provides a new level of total automation on agarose gel.

You simply pipette the hemolysates into the sample wells, put the gels in the holders, start the instrument and walk away!

Synthesis of hemoglobin is under genetic control and the presence of abnormal hemoglobins is associated with functional, physical and morphological abnormalities of the red cells.
Hemoglobins are composed of polypeptide chains of globin and iron protoporphyrin heme groups.
Normal hemoglobins have similar structures with a molecular weight of about 67,000 daltons and consist of 2 pairs of globin chains each associated with one molecule of heme.
Hemoglobin A polypeptide chains 2

and 2

Hemoglobin A₂ polypeptide chains 2

and 2

Hemoglobin F polypeptide chains 2

and 2

Normal adult hemoglobins are HbA (98% of total hemoglobin) and small amounts of HbA₂ and HbF. Substitution of aminoacids in the polypeptide chains sequence leads to the formation of abnormal hemoglobin variants. At present over 600 structural hemoglobins variants are known: HbS, HbC, HbE, HbD, HbG, HbH, HbI, Hb Lepore, etc.
Hemoglobinopathies are a group of diseases caused by the presence of abnormal hemoglobins.
About 60% of abnormal hemoglobins have a sufficiently altered charge distribution to be identified by electrophoresis. The variants most frequently observed are HbS and HbC.
Concomitant presence of HbA and another variant (HbS, HbC, HbE, etc.) is referred to as heterozygous hemoglobinopathy AS, AC and AE respectively.
The presence of only one single type of hemoglobin variant is called homozygous hemoglobinopathy, like HbS or HbC.
These abnormal variants are genetically transmissible. Homozygous hemoglobinopathies may cause serious clinical effects. Acid hemoglobin electrophoresis on agarose allows confirmation of the presence of HbS or HbC already detected by Alkaline Hemoglobins electrophoresis.

Kit Content

Gel Plates	10
Blotting Paper	10
Buffered Sponges	20
Amido Black Stain	1
Applicator Washing Solution	1
Lysing Solution	1
Disposable Sample Plates	10

Regents preparation

Reagents are ready to use, only the Stain has to be reconstituted with 900 ml of distilled water, all may be stored at room temperature.

Samples preparation

After the red blood cells (RBC) are washed they are lysed as follows: 50 µl of packed washed RBC + 450 µl of lysing solution.

Sample storage and stability

Whole blood:
Hemolysate:

1 week at 2 to 8°C
12 hours at 2 to 8°C

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