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Alkaline Hemoglobins Electrophoresis Kit

The Microgel/Interlab G26 H.R. Alkaline Hemoglobins Electrophoresis method provides a new level of total automation on agarose gel.

You simply pipette the hemolysates into the sample wells, put the gels in the holders, start the instrument and walk away!

	In the red blood cells of a normal adult three different types of hemoglobin exist. HbA is the major type with small amounts of HbA2 and HbF. The two mutant hemoglobins most commonly seen are HbS and HbC (Hb Lepore, Hb Bart’s, HbH, HbE, HbG-Philadelphia, HbD-Los Angeles, and HbO-Arab may be seen less frequently).
	The alkaline hemoglobins electrophoresis test system is intended for the separation of normal hemoglobins (HbA and HbA2) as well as certain abnormal or variant hemoglobins (HbS or HbD, and HbC or HbE). However, some abnormal and normal hemoglobins may co-migrate (for example, HbA2 and HbC; and HbS and HbD).
	Hemoglobins with identical mobility can be differentiated by hemoglobin electrophoresis test run at acid pH. Acid hemoglobins electrophoresis allows separation of these hemoglobins to confirm the identity of clinically relevant hemoglobins such as HbA, HbF, HbS and HbC.
	Hemoglobinopathies comprise a large number of hereditary disorders that can produce either qualitative modification of hemoglobin structure, or quantitative variations of hemoglobin synthesis, this eventually leads to an imbalance of the normal concentration of the different types of hemoglobins.
	The term Thalassemia refers to a group of diseases (also called syndromes) with similar clinical effects, whose main biochemical feature is the imbalance between the synthesis rate of one type of globin chain and that of its mate. Globin chain imbalance damages and destroys red cells leading to anemia.

Kit Content

Gel Plates	10
Blotting Paper	10
Buffered Sponges	20
Amido Black Stain	1
Applicator Washing Solution	1
Lysing Solution	1
Disposable Sample Plates	10

Regents preparation

Reagents are ready to use, only the Stain has to be reconstituted with 900 ml of distilled water, all may be stored at room temperature.

Samples preparation

Neat urine. Diluted serum 1:20. Concentrated CSF to a final total protein concentration of 10 g/L.

Sample storage and stability

Serum:	1 week at 2 to 8°C, 1 month at -20°C
Urine:	1 week at 2 to 8°C, 1 month at -20°C
CSF:	1 week at 2 to 8°C, 1 month at -20°C

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