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The Microgel/Interlab G26 Immunofixation Electrophoresis (IFE) kit, thanks to the easy mask, provides a semi automated method for detection and identification of suspected monoclonal components.
The first 8 Immunofixation samples are completed within 40 minutes and subsequent groups every 15 minutes. Microgel and Interlab G26 work by a continuous loading mode.
No sample dilution!
1. Pipette the specimens into the sample wells
2. Place gels into the holder
3. Start the instrument
4. WALK AWAY!
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Monoclonal immunoglobulins are due to malignancies of B lymphocyte
origin. The principle of immunofixation electrophoresis is based
on the visualization of specific proteins through antigen-antibody precipitation,
following protein separation by electrophoresis.
Patient specimens are placed into the sample wells and the major protein
groups are then separated by electrophoresis.
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For each Immunofixation sample six patterns are obtained on the gel plate.
One pattern is treated with a fixative solution to create a serum proteins electrophoresis (SPE) reference pattern.
Antisera with specificities for the heavy chains, anti gamma (IgG), anti alpha (IgA), anti mu (IgM) and for the light chains and (free and bound) are applied on the other five patterns.
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Kit Content |
Gel Plates |
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Blotting Paper A |
30 |
Blotting Paper L |
10 |
Blotting Paper G |
10 |
Buffered Sponges |
20 |
| Acid Blue Stain |
1 |
Applicator Washing
Solution |
1 |
Washing Solution 1 for
Immunofixation |
1 |
Washing Solution 2 for
Immunofixation |
1 |
| Immunofixation Diluent |
1 |
| Disposable Sample Plates |
10 |
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Regents preparation |
Reagents are ready to use, only the Stain and the Washing Solution for Immunofixation have to be reconstituted:
- Reconstitute Stain with 900 ml of distilled water.
- Dilute 20 ml of Washing Solution for Immunofixation to a final volume of 1L with distilled water
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Samples preparation |
Neat serum samples. Concentrated urines to a final total protein value of about 5 g/L
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Sample storage and stability |
| Serum: |
3 days at 2 to 8°C |
| Urine: |
1 week at 2 to 8°C, and 1 month at 20°C |
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