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The Microgel/Interlab G26 LDH Isoenzymes Electrophoresis kit, thanks to the easy mask, provides a semi-automated method for detection of LDH Isoenzymes. The kit is extremely user friendly and easy to use! In 1 hour and 5 minutes the first 26 LDH samples are completed and subsequent groups every 20 minutes.

1. Pipette the specimens into the sample wells
2. Place gels into the holder
3. Start the instrument
4. WALK AWAY!

Lactate dehydrogenase (LDH, E. C. 1.1.1.27.) is an intracellular enzyme found in human tissues, such as skeletal muscle, heart, liver and kidneys. The molecule is composed of two subunits, H and M, that are assembled to give a tetramer. Different qualitative association of the subunits results in the formation of five different LDH molecules called ‘isoenzymes’ or ‘isoforms’, that can be separated by electrophoresis on agarose gel. According to the electrophoretic mobility five bands can be separated. The LDH1 isoform, found in heart muscle, is composed of four H subunits and is the most anodic, while the most cathodic isoform is LDH5 that is found in skeletal muscle and liver, and is composed of four M subunits. Isoenzymes of intermediate mobility, LDH2, LDH3 and LDH4, are found in varying degrees in many tissues. Quantitation of these fractions provides useful information on the identification of specific tissue or organ damage. Electrophoresis of LDH isoenzymes is an important test for the diagnosis of myocardial and liver damages. Marked increase of LDH1 is observed after myocardial infarction (MI), resulting in a LDH1/LDH2 ratio greater than 1 (‘flipped LDH’). LDH5 is an excellent indicator of active liver damage. Interfering substances : Do not use hemolyzed samples and uremic sera. Some drugs and inhibitors (like oxalate) affect the activity of LDH. Repeated freezing and thawing of samples destroys LDH activity