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Lipoproteins Electrophoresis Kit

The Microgel/Interlab G26 Lipoprotein Electrophoresis method provides a new level of total automation on agarose gel.

You simply pipette the specimens into the sample wells, put the gels in the holders, start the instrument and walk away!

Lipids display a number of different and important roles in metabolic processes, they serve as hormones, they participate in digestion, act as an energy store, can be used as metabolic fuel and are important components of biological membranes.
Correlation between plasma lipid abnormalities and atherosclerosis have been established.
Lipoproteins are macromolecular complexes composed of lipids and proteins.
Lipoproteins are spherical particles consisting of a core of non polar lipids surrounded by polar lipids and one or more proteins, the apoproteins, at the surface. The core contains triglycerides, cholesterol esters, phospholipids and free cholesterol. The different proportions in the lipoprotein complexes of proteins and lipids allow their separation by electrophoresis on agarose gel, based on differences in their net charge.
At alkaline pH four bands can be resolved: alpha-lipoprotein, pre-beta-lipoprotein, beta-lipoprotein and chylomicrons.
Disorders of lipoproteins metabolism comprise a variety of conditions including hyperlipoproteinimias due to increased production of lipoproteins. They have been classified according to Fredrickson and co-workers into five main groups, each associated to an electrophoretic pattern and graph. Interpretation of these profiles is important for the management of hyperlipoproteinemias.
Interfering substances:
EDTA activates lipases and should be avoided.

Kit Content

Gel Plates	10
Blotting Paper	10
Buffered Sponges	20
Sudan black stain	1
Applicator Washing Solution	1
Disposable Sample Plates	10

Regents preparation

Reagents are ready to use, only the Stain has to be reconstituted by mixing 5 ml of concentrated Sudan Black solution with 450 ml of ethanol + 450 ml of distilled water.

Samples preparation

Fresh neat serum samples.

Sample storage and stability

Serum:

2 day at 2 to 8°C tightly capped.

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