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Protein Electrophoresis kit Serum Proteins and Concentrated Urines

The Microgel/Interlab G26 Serum Protein/Concentrated Urine Electrophoretic methods, provide a new level of total automation on agarose gel.

You simply pipette the specimens (pure or concentrated) into the sample wells, put the gels in the holders, start the instrument and walk away!

Human body fluids contain a varied mixture of proteins and protein complexes. Each of these protein entities apparently fulfills a specific function in physiological process; furthermore, it is well known that the levels of various proteins in blood serum bear a close relationship to states of health and disease. Of the many techniques available for separating proteins, electrophoresis is a well established and versatile technique, routinely used in clinical laboratories. The most popular method of electrophoretic protein analysis is “zone electrophoresis” on agarose gels. Serum Protein Electrophoresis (SPE) performed at pH 8.9 yields five bands: albumin and four globulins (each fraction containing a number of different proteins): alpha 1(

1), alpha 2 (

2), beta (

), and gamma (

). Evaluation of single bands by visual inspection provides valuable diagnostic support as it offers a display of the major proteins involved in functional and pathological processes.

The appearance of plasma proteins in the urine (proteinuria) is of great value in the evaluation of renal function. The physiological proteinuria is defined as approximately 150 mg of protein found in a 24 hour urine collection.
Electrophoresis of urine is the best way to detect abnormal proteins in urine.
The presence of an abnormal band requires complementary identification by Immunofixation (for example to assess the presence of a Bence-Jones protein) Abnormal proteins in urine can be present with a normal proteinuria.

Kit Content

Gel Plates	10
Blotting Paper	10
Buffered Sponges	20/30
Amido Black Stain	1
Applicator Washing Solution	1
Disposable Sample Plates	10

Regents preparation

Reagents are ready to use, only the Stain has to be reconstituted with 900 ml of distilled water. All may be stored at room temperature.

Samples preparation

Neat serum samples. Concentrated urines to a final total protein concentration ≥ 20 g/L.

Sample storage and stability

Serum:	3 days at 2 to 8°C
Urine:	1 week at 2 to 8°C, and 1 month at –20°C

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